NOVEL NEXT Chapter 102 Inducing super bacteria!
Lu Liang soon received the sample sent by Tian Li.
After opening the courier, Lu Liang discovered that Tian Li had sent him a large package of drug samples.
It's like it's free of charge.
Lu Liang stored the sample in a black opaque bag, stuffed it into a 4°C refrigerator, and then began to prepare for cultivating bacteria.
The first thing before cultivating bacteria is to brush the bottle.
Lu Liang counted all the petri dishes and Erlenmeyer flasks in the laboratory. There were quite a few petri dishes and bottles to be used.
Seeing a basin full of petri dishes, Lu Liang couldn't help but sigh. This was a big project to paint.
If you do it alone, you don’t know how long it will take.
However, maybe someone else can be found to help, a figure appeared in Lu Liang's mind.
In the study room on the second floor, Wen Yong leaned on his chair and looked out the window boredly.
The failure of yesterday's test made Wen Yong a little frustrated now.
At this time, a girl came over and asked cautiously: Brother, aren't you going to do a test today?
Wen Yong replied lazily: I don't want to do it. If I do it, it will be a failure anyway. It's better not to do it.
The girl had a troubled look on her face: Aren't you afraid that the teacher will criticize you?
It doesn't matter, I'm thick-skinned anyway.
Wen Yong replied nonchalantly that since he became a veteran, he has long been used to this kind of thing.
The girl opened her mouth and wanted to say something.
But he was interrupted by Wen Yong: I left my words here. Even if the King of Heaven comes today, it is impossible for me to step into the laboratory.
After seeing Wen Yong's resolute attitude, the girl could only shut up angrily and give up the idea of letting Wen Yong teach her to do experiments.
Just when the girl was about to leave, the door of the study room was suddenly pushed open.
Wen Yong and the girl followed the call and saw Lu Liang pushing open the door of the study room and walking in.
After meeting Wen Yong, Lu Liang asked: Brother Yong, can you help me do an experiment?
The girl just wanted to help Wen Yong refuse, but before she could say anything, Wen Yong stood up with a snap: No problem, let's go.
Ever since he tasted the sweetness last time, Wen Yong has always been positive about this kind of thing.
At this moment, Lu Liang also noticed the girl standing next to Wen Yong. He asked tentatively: I may need some time here, so it won't delay your business, right?
Don't worry, I have a lot of time. I'm worried that I have nothing to do. Even if the King of Heaven comes today, he can't stop me from entering the laboratory.
The frustration on Wen Yong's face had long been wiped away, and he followed Lu Liang's footsteps with excitement.
Seeing Wen Yong's excited departure, a big question mark appeared in the girl's mind. This was completely different from what she just said!
In the microbiology laboratory, Wen Yong cleaned the petri dish, then turned to look at Lu Liang and asked curiously.
Brother Lu, are you planning to grow bacteria and wash so many petri dishes?
Yes, this time raising bacteria.
Lu Liang nodded, and then continued to use the weighing scale to weigh out what he needed for the culture medium.
Wen Yong came over curiously, pointed to the white powder Lu Liang was weighing and asked, Brother Lu, what is this?
Common antibiotics.
Lu Liang replied that this was also part of the raw materials he needed for his culture medium.
antibiotic?
Wen Yong frowned in confusion.
He knew very well the configuration of bacterial culture media.
Because the bacteria cultured are different, the media prepared are also different.
For example, for anaerobic bacteria, an appropriate amount of reducing agent needs to be added to the culture medium to reduce the redox reaction in the culture medium, which is beneficial to the production of anaerobic bacteria.
But the question is, what kind of bacteria do you need to use antibiotics as a culture medium?
Aren't all the bacteria killed? How about cultivating them?
Suddenly, Wen Yong seemed to realize something, and his pupils shrank suddenly.
Indeed, under normal circumstances, bacterial culture media do not require the use of antibiotics.
There is one special exception.
In that particular case, different concentrations of antibiotics are added to the culture medium.
That is, inducing super bacteria!
Hiss~!
Wen Yong took a breath: Brother Lu, you don't want to...
Well, induce superbugs.
Lu Liang nodded and answered calmly.
He needs to prove that the third generation of evil has an antibacterial effect on super bacteria, so naturally he cannot use ordinary bacteria for the test.
Therefore, the first step is to induce ordinary bacteria into super bacteria.
Then you have to be careful not to take it out of the laboratory. Wen Yong reminded.
Don't worry, I understand the process. Lu Liang said while pouring the weighed dry agar powder into an Erlenmeyer flask, adding distilled water to dissolve it, and sealing it tightly with kraft paper.
After doing all this, the next step is sterilization.
All instruments require strict sterilization.
Otherwise, the bacteria grown will always be miscellaneous bacteria.
After stacking the petri dishes that Wen Yong washed one by one, Lu Liang put the petri dishes and the prepared culture medium into the high-pressure steam sterilization pot.
After putting the lid on the pot and tightening the valve, Lu Liang checked twice carefully.
After all, he had encountered an accident before where someone failed to tighten the valve, causing the pot lid to be blown open by the steam pressure.
That movement is still fresh in Lu Liang's memory.
Because the high-pressure steam cooker was in the laboratory next to him at that time, and it was directly used for other people's anesthesia.
After confirming that nothing was missing, Lu Liang started the sterilizing pot.
Half an hour passed. After completing the sterilization, Lu Liang took the things to the ultra-clean table.
Next, it’s time to induce superbugs!
At the same time, some indescribable activities began in the microscopic world invisible to the naked eye.
Staphylococcus aureus settled on a ground full of gelatin.
After arriving in this strange place for the first time, Staphylococcus aureus showed strange confusion.
After all, he had been frozen in a deep sleep in a -80°C refrigerator before. He only woke up from his deep sleep after feeling that the ambient temperature was suitable.
And he was surrounded by fellow members of his race who had just woken up like him.
However, Staphylococcus aureus quickly responded. It didn't matter where it was. What was important was whether this place was suitable for survival and reproduction.
After all, survival and reproduction are the first instructions engraved in their DNA.
Staphylococcus aureus Check the environment immediately.
Suitable temperature, suitable PH value, abundant water, abundant carbon sources, abundant nitrogen sources, and abundant inorganic salts
This is
Staphylococcus aureus is a little excited, this is a fertile soil that has never been seen before!
Breed, brothers!
Following the orders of Staphylococcus aureus, they began to mine the materials they needed, and they reproduced from generation to generation, forming huge colonies one after another.
Looking at the growing population, Staphylococcus aureus showed a happy smile.
But the good times did not last long. As the number of ethnic groups increased exponentially, the nutrients in this land could no longer support his colony.
If this continues, his tribe will surely die.
The only way is to expand the territory.
Thinking of this, Staphylococcus aureus showed sadness on his face, and he frowned and looked at the dark land in the distance.
It was a land full of death and ominous atmosphere, and all Staphylococcus aureus that set foot on that land died inexplicably.
This made them full of fear and fear of that land, and all Staphylococcus aureus shunned that land.
But now, in order to survive, they must set foot on that land.
Staphylococcus aureus brings its tribe together.
Brothers, our tribe has now reached a critical moment of life and death!
If we can no longer find new nutrition, our tribe will surely perish, so we must now set foot on that land full of death.
I know that once we set foot on that land, we will all die, but we are not afraid, we are bacteria!
We have lived in this world for 3.5 billion years. We have witnessed the Ordovician period, the Cretaceous period, the fossilization of trilobites, and the mass extinction of the dinosaurs.
But we still survived. Nothing in this world can exterminate us, not before, not now, and never in the future!
So, for the sake of the colony, brothers, rush with me!
As Staphylococcus aureus finished speaking, other Staphylococcus aureus roared and followed suit.
And the moment Staphylococcus aureus set foot on the black soil, the breath of death rushed towards us.
Soon, Staphylococcus aureus fell down one after another, but there were still countless Staphylococcus aureus rushing up behind them.
At this moment, after seeing his body begin to collapse, a keen Staphylococcus aureus finally realized the cause of death.
At the last moment when the body was broken, the golden Staphylococcus aureus roared out with its last strength.
Antibiotics, antibiotics, mutate, mutate quickly!
After being yelled at by that Staphylococcus aureus, the other Staphylococcus aureus reacted one after another.
They began to change their DNA. They rushed up again and again, died again and again, and modified their DNA again and again.
Finally, the first Staphylococcus aureus bacteria, unaffected by antibiotics, stood on the black soil.
Look, it's drug resistance, he has developed drug resistance!!
There’s Staphylococcus aureus yelling.
This scene immediately boosted the morale of other Staphylococcus aureus bacteria, and they modified their DNA to look like that Staphylococcus aureus bacteria.
As a result, more and more strains of Staphylococcus aureus became resistant, and they roared onto the land.
This time, the antibiotics couldn't kill them.
But the pace of Staphylococcus aureus did not stop there.
Because there is darker land behind, where there are higher concentrations of antibiotics!
But this is of no use to them. They just need to continue to improve their drug resistance.
Finally, after all the land was covered with traces of Staphylococcus aureus, the Staphylococcus aureus embraced each other passionately, and they won the battle.
They conquered death!
call!
In the laboratory, Lu Liang, who was under tight protection, breathed a sigh of relief.
Generally speaking, obvious mutations will occur after gene accumulation for about 100 generations, and it only takes 20 minutes for bacteria to reproduce once.
When deliberately cultivated, bacteria can quickly evolve resistance and grow on those media containing antibiotics.
Therefore, using low concentrations of antibiotics to induce superbugs is a commonly used method in the laboratory.
And now, the super bacteria has been induced successfully!